affinity column meaning in Chinese
亲和层析柱
亲和柱
Examples
- Were passed through affinity column , separated by sds - page and stained using con a - hrp
用cona - hrp检测获得分子量为37和39kd两种糖蛋白特异成分。 - After the protein refolding of denaturant inclusion body following dialysis , we got the pure recombinant gst - eo protein by gst affinity columns . using the purified protein as coating antigen , an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum
使用分步透析法对变性的包涵体进行复性,将复性蛋白过gst亲和层析柱得到纯化的gst - eo融合蛋白。以gst - eo融合蛋白为诊断抗原,初步建立了用间接elisa检测猪瘟血清eo抗体的方法。